Colocalization without software?

2With the widely use of confocal microscopes, scientists are revealing ineteresting interactions between molecules. “Cololization” seems to be the buzz word. However, imaging processing is somehow not something just as you see, i.e. it is not WYSIWYT (what you see is what you think). It is more or less beyond the curriculum of many biologists. Many of my coleagues act in a way that if the two channels have signal in the same region to give another color, that is colocalization. In fact this is a false conclusion. Although confocal microscopy is really powerful, images still are far beyond a direct “yes” or “no”. On the mailing list of CONFOCAL, someone started such a “troll”, and many valuable info is in the thread. Check it out before you do the experiments.